RpoS-Regulated Genes of Escherichia coli Identified by Random lacZ Fusion Mutagenesis
نویسندگان
چکیده
منابع مشابه
RpoS-regulated genes of Escherichia coli identified by random lacZ fusion mutagenesis.
RpoS is a conserved alternative sigma factor that regulates the expression of many stress response genes in Escherichia coli. The RpoS regulon is large but has not yet been completely characterized. In this study, we report the identification of over 100 RpoS-dependent fusions in a genetic screen based on the differential expression of an operon-lacZ fusion bank in rpoS mutant and wild-type bac...
متن کاملAnaerobically expressed Escherichia coli genes identified by operon fusion techniques.
Genes that are expressed under anaerobic conditions were identified by operon fusion techniques with a hybrid bacteriophage of lambda and Mu, lambda placMu53, which creates transcriptional fusions to lacZY. Cells were screened for anaerobic expression on XG medium. Nine strains were selected, and the insertion point of the hybrid phage in each strain was mapped on the Escherichia coli chromosom...
متن کاملExpression Cloning of Recombinant Escherichia coli lacZ Genes Encoding Cytoplasmic and Nuclear P-galactosidase Variants
Objective(s) Nonviral vector can be an attractive alternative to gene delivery in experimental study. In spite of some advantages in comparison with the viral vectors, there are still some limitations for efficiency of gene delivery in nonviral vectors. To determine the effective expression, the recombinant Escherichia coli lacZ genes were cloned into the different variants of pcDNA3.1 and the...
متن کامل-regulated genes in Escherichia coli
Accepted 9 March, 2006. *For correspondence. E-mail pjkiley @wisc.edu; Tel. ( + 1) 608 262 6632; Fax ( + 1) 608 262 5253. Present address: † The Burnham Institute, La Jolla, CA 92037, USA; ‡ Department of Biomolecular Chemistry, University of Wisconsin, Madison, WI 53706, USA. IscR-dependent gene expression links iron-sulphur cluster assembly to the control of O 2 -regulated genes in Escherichi...
متن کاملPenicillin-binding proteins are regulated by rpoS during transitions in growth states of Escherichia coli.
Attention has been recently focused on the role of the rpoS (formerly katF) gene product as a regulator during the transition from the exponential growth phase to the stationary phase as well as during nutritional starvation. It has been demonstrated that RpoS is an alternate sigma factor which would bind to promoters of genes induced at these times. It was previously noted that rpoS mutants do...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2004
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.186.24.8499-8507.2004